An Assessment of the Baxx Device

An Assessment of the Baxx Device

The Baxx device

1.0 This report returns the results of initial tests conducted with a device called the Baxx system. The Baxx device is intended to operate in a manner which constantly delivers a Hydroxyl cloud capable of achieving both sanitizing effects and odour control. The device assessed in this study is intended for treating spaces and surfaces of up to 250 m3.

The objectives of this trial were as follows ;

a) To demonstrate the efficiency of the device in reducing microbial contamination on solid surfaces during continuous usage.

b) To demonstrate the efficiency of the device in the reduction of atmospheric levels of microorganisms during continuous usage.

c) To demonstrate the impact on the rancidity characteristics of whole birds (p+1 ) under continuous exposure at 5’c

d) To demonstrate any reduction in microbial contamination of whole birds (p+1 ) under continuous exposure at 5’c

2.0 Method Outline

2.1 Assessment of the reduction of microbial contamination on solid surfaces ;

This experiment was conducted in two 250 m3 laboratory spaces. During the trial area A was continuously treated with the Baxx device while area B was not treated The microbial challenge consisted of plastic templates ( 700 cm2) which were divided into a series 25 cm2 sectors and which were seeded with individual microbial cultures dispersed in Brain heart infusion. The surfaces were allowed to dry prior to the trial. Six templates for each organism were distributed randomly in each of the test environments

The following organisms were employed ;

Salmonella seftenberg
Staphylococcus aureus
Pseudomonas aeruginosa
Aspergillus niger
Listeria monocytogenes
Escherichia coli

On each day of the trial random plastic pieces were selected in each environment for each organism and the viable number of organisms for duplicate 25 cm2 was determined after recovery by swabbing followed by serial dilution and analysis by spiral platting on appropriate microbiological media.

In this manner any effect antimicrobial produced by the Baxx treatment could be assessed by comparison the result obtained in an untreated environment

2.2 Assessment of reduction in Atmospheric contamination

This experiment was conducted in a waste room situated in a microbiological laboratory. This environment is intended to store bags of Class I and Class II waste intended for sterilisation by autoclaving. Due to the nature of the work carried out in this environment there is possibility of atmospheric contamination by microorganisms.

The quality of the atmosphere was assessed by volumetric sampling of a 50 Litre volume four times per day ( 0800, 1200, 1600 & 2000 hrs) employing an impaction air sampler. The quantities determined were aerobic plate count , Yeast count and the Mould count. The results of these determinations are expressed as mean count per Litre of atmosphere per day over the trial period.

Control sampling was conducted over a seven day period prior to Baxx treatment and additionally over a further seven day period with the Baxx device running continuously. The volume of the room was 70 m3.

By comparing mean counts for the measured parameters throughout the untreated and treated period an estimate of atmospheric microbial reduction has been achieved.

2.3 Assessment on the impact on rancidity and microbial reduction characteristics of Free range whole birds (p+1 ) under continuous exposure to Baxx treatment at 5’c .
This series of experiments was conducted in two chiller units (134 m3). 12 birds were located in each chiller. Chiller A was untreated while chiller B was exposed to continuos dosing by operation of the Baxx device over a 12 hour period.
During the trial period duplicate samples of breast skin were removed from each population on an hourly basis. The fat from these samples was extracted in the dark with cold Chloroform and subsequently the F.F.A. and P.V. values were determined.
Sub samples of skin were examined to determine the levels of Pseudomonas sps, Yeasts and Campylobacter sps.

3.0 Results section

Results for the reduction of organisms on plastic surfaces due Baxx treatment

Table 1 : Survival characteristics of microbial cultures on plastic surfaces situated in a 250 M3 space during continuous treatment with Baxx device.

Results for the Atmospheric reduction of organisms due to Baxx treatment

Results for Rancidity effects on whole Birds Bird Breast Skin due to Baxx treatments

Results for reduction of microbial contamination in Whole Birds Bird Breast Skin due to Baxx treatments

Conclusions

Consensus opinion in the literature supports a theory that Hydroxyl dosing of microorganisms leads to lethality due to irreversible inactivation of essential enzymes.

During this trial we have shown that over a seven day period the Baxx device during continuous operation will produce between a Log 4 and Log 5 reduction of common food spoilage organisms and pathogens on the surface of plastic templates.

Additionally our work indicates that in an area subject to frequent recontamination by microbiological waste the Baxx device is capable of delivering a constant 2 log improvement in atmospheric levels of Total viable count and Fungi ( Yeasts and Moulds).

Further by exposing whole birds to dosing with the Baxx device we have been able to demonstrate significant reduction in breast skin contamination by Pseudomonas species ( 2.7 Log ) , Campylobacter species (1.7 Log) and Yeasts ( 1.8 Log)

On the basis of these results there exists a strong case that microbiological quality of food production environments could be improved by regular prolonged or continuous dosage with the Baxx unit.

Our results for the impact of prolonged ( 12 hours) Baxx treatment in relation to the development of rancidity in the breast skin of free range whole birds indicates that after 10 hours of dosing there is a significant upward trend in Peroxide value . ( Treated after 12 hours 5.0 Untreated after 12 hours 2.0) . A similar trend in F.F.A was not observed.

My opinion is that further work across several batches of different types of birds is required to establish definitively the impact of long term( greater than 10 hours) Baxx dosing of primary materials with regard to rancidity and organoleptic effects.